Hans J. Vogel

Professor of Biochemistry, AHFMR Scientist,
Department of Biological Sciences,
The University of Calgary
Phone:(403) 220-6006 
Fax: (403) 289-9311
email: vogel@acs.ucalgary.ca



EXPERIMENTAL TECHNIQUES USED

NMR SPECTROSCOPY

-multinuclear one-dimensional NMR (1H, 13C, 15N, 19F, 31P, 77Se)
-quadrupolar metal ion nuclei (27Al, 43Ca, 45Sc, 51V, 71Ga)
-spin 1/2 substitute metal ions (113Cd, 207Pb)
-heteronuclear and homonuclear two-and three-dimensional NMR
-solid state NMR (structure and dynamics)
-NMR determinations of protein pKa's
-surface exposure measurements

EPR SPECTROSCOPY

-nitroxide spin labeling of peptides and proteins
-orientation of peptide to protein binding
-NMR/EPR distance measurements

BIOCOMPUTING (Collaborators: Berendsen, van der Spoel, Mavri)

-molecular and essential dynamics calculations
-theoretical pKa calculations of proteins
-homology modeling of related proteins
-quantum chemistry calculations of amino acids and their interactions

BIOCHEMICAL APPROACHES

-protein purification and cloning
-protein overexpression
-enzymatic assays
-gel band shift assays
-incorporation of unnatural amino acids
-specific isotope label incorporation
-site-directed mutagenesis
-limited proteolysis
-chemical modification

FLUORESCENCE SPECTROSCOPY

-tryptophan fluorescence
-selenomethionine quenching, peptide to protein orientation
-fluorophenylalanine containing proteins

INFRARED SPECTROSCOPY (collaborators, Fabian, Mantsch)

-secondary structure of peptides and proteins
-isotope labeling effects on infrared spectra
-unnatural amino acid incorporation in proteins
-protein-peptide, protein-protein interactions

CIRCULAR DICHROISM

-secondary structure of peptides and proteins (far UV)
-aromatic amino acids environment (near UV)

ULTRAVIOLET SPECTROSCOPY

-protein purity; protein denaturation
-incorporation of fluorinated aromatic amino acids

X-RAY CRYSTALLOGRAPHY(collaborators Phipps, Tari)

-calmodulin variants; mutants and unnatural amino acids
-calmodulin like domain of CDPK's structure determination
-bacterial periplasmic iron binding proteins.

SMALL ANGLE X-RAY SCATTERING (collaborator Shilton)

-structural changes in periplasmic iron binding proteins

MASS SPECTROMETRY OF PROTEINS (collaborators Olafson, Lajoie)

-analysis of mutant proteins, and proteins with unnatural amino acids
-calmodulin-peptide binding studies

{ Research | Selected Publications | Selected Review Papers | Experimental Techniques Used }
{ Proteins Studied | Career Highlights | Pictures of Favourite Proteins | Hobbies }
{ University of Calgary Bio-NMR Center }